Multiomics
Single Cell
Single-cell Whole Genome and RNA Sequencing
Experience the power of single-cell multi-omics, by performing Single-cell Whole Genome Sequencing (scWGS) and Single-cell RNA sequencing (scRNA-seq) on the same cell (scWGS+scRNAseq).
With Primary Template-directed Amplification (PTA) as the whole genome amplification (WGA) method, scWGS can be performed with reduced amplification artifacts, high genomic uniformity and low error propagation.
Mirxes Genomics offers scWGS+scRNAseq as an end-to-end service, supporting seamless integration of genomic variation data with transcriptional information to generate unparalleled insights into the drivers and consequences of cell heterogeneity.
Integrate Genomic and Transcriptomic Insights from the Same Cell
Partner with Us to Achieve Your Research Goals
Single-cell Multi-omics
Whole genome and mRNA transcriptome information obtained from the same cell
Exceptional WGA Performance
High genome coverage and uniformity, low error and reduced artifacts
Full Gene Body Coverage
Excellent detection of
full-length mRNA compared to 3’ scRNA-seq methods
Expert Bioinformatics Support
Bioinformatic heavy lifting of huge multi-omics datasets
Isolated single cell
Cytosolic lysis and reverse transcription
Nuclear lysis and whole genome amplification
Affinity separation and amplification of cDNA pool
Library construction and sequencing
Bioinformatics analysis
| Recommended sequencing depth* | Sequencing Platforms | Turnaround Time |
|---|---|---|
| 300M reads for DNA; 1M reads for RNA | DNBSEQ-T7 or DNBSEQ-T10 | 4-6 weeks from successful WGTA QC* to FASTQ file delivery |
*This serves only as a guide. Please contact us for further discussion.
| Sample Type | Sample Source | Brief Sample Preparation Guideline* |
|---|---|---|
| Single intact (viable) cells | Fresh tissue, Cell line | - Single cells should be dispensed into 96-well plate (Lobind) - After cell dispensing, the plate should be spun down and stored at -80°C until use - Note: DAPI staining is not compatible with this assay |
Kindly note that this serves as a brief reference only.
* Please contact us for the complete sample preparation and submission instructions.
