Multiomics
Bulk
Shotgun Metagenomic Sequencing
Shotgun Metagenomic Sequencing involves untargeted sequencing of all microbial genomes in each sample, enabling unbiased characterization and in-depth study of microbial communities in their living environments. Discover the diversity and functional potential of microbiome, which plays a major role in human health and disease.
Mirxes Genomics offers high throughput Shotgun Metagenomic Sequencing, complete with Bioinformatics analysis customized to meet your specific project requirements.
Untargeted Sequencing
Unbiased characterization of microbiome
Study Host-Microbiome Interactions
Both host and microbial genomes are sequenced and available for analysis
Expert Bioinformatics Support
Custom analysis, with guaranteed data security
High Sequencing Throughput
High-capacity sequencers to generate more data for insightful analysis
Extraction
Library
preparation
Sequencing
Bioinformatics analysis
| Minimum Data Quantity (before host genome exclusion) | Sequencing Platform | Turnaround Time |
|---|---|---|
| Saliva: ≥ 10 Gb raw data | DNBSEQ-T7 | 2-4 weeks from successful sample QC* to data delivery |
| Skin: ≥ 10 Gb raw data | ||
| gDNA from other sources e.g. Stool: ≥ 6 Gb raw data |
*The sample QC requirement for this assay is dependent on the assay type.
Note: Minimum data quantity required may vary depending on the source of the gDNA, and the purpose of the sequencing project. Please contact us for further recommendations of sequencing data quantity.
| Sample Type (pre-extraction) | Optimal Input Amount |
|---|---|
| Human skin (skin swab or tape) | Variable depending on sampling method, please contact us with the specific request so that we may advise accordingly |
| Human saliva | ≥ 2 ml |
| Sample Type (post-extraction) | Optimal Input Amount | Volume | Concentration | Purity and Quality |
|---|---|---|---|---|
| Human gDNA | ≥ 100ng | ≥ 20μl | ≥ 5 ng/μl |
OD260/280 = 1.8 - 2.0 OD 260/230 ≥ 1.7 DIN ≥ 7 |
Please note that these requirements serve only as a guide. Please contact us for further assessment if your samples do not meet the requested amounts.
*Our Bioinformatics team may provide recommendations based on your specific project goals and samples
- Data Quality Control: Filtering reads with adapter or low-quality sequence data
- Removal of host genome
- Taxonomy and abundance
- Gene function annotation
- Pathway analysis for different taxa identified
- Linkage between microbial taxa and specific variations in human genome
- Variation calling from whole genome
- Antibiotic Resistance Gene Annotation
- Other options available upon request