Multiomics
Bulk
Whole Genome Sequencing
Whole Genome Sequencing (WGS) enables comprehensive characterization of the entire human genome, including coding and non-coding regions.
Mirxes Genomics utilizes unique DNA nanoball sequencing (DNBSEQ) technology coupled with PCR-free library preparation to deliver results with high accuracy and low sequencing artifacts. Sequence alignment and variant calling are also available for our WGS services.
PCR-free Library
Minimize errors and amplification bias
High Sequencing Capacity
Up to 640 genomes in one run
High Quality Data
Q30 score of >80% for PE150 and >85% for PE100
Expert Bioinformatics Support
Custom analysis, with guaranteed data security
PCR-free library preparation
Sequencing
Bioinformatics analysis
| Suggested Sequencing Depths | Sequencing Platform | Turnaround Time |
|---|---|---|
|
Rare diseases 30x (90Gb) |
DNBSEQ-T7 or DNBSEQ-T10 |
2-4 weeks for T7 3-6 weeks for T10 from successful sample QC to data delivery |
|
Oncology Tumor: ≥50x (≥150Gb) Adjacent or blood normal: 30x (90Gb) |
| Service | Sample type | Sample Source | Optimum Input Amount | Volume | Concentration | Purity and Quality |
|---|---|---|---|---|---|---|
| hWGS (PCR-free) | gDNA | Fresh frozen tissue, Cell line, Saliva, Blood | ≥ 1.5μg | ≥ 20μl | ≥ 20ng/μl | OD260/280 = 1.8 - 2.0 OD 260/230 ≥ 1.7 DIN ≥ 7 |
| hWGS (PCR-based) | gDNA | Fresh frozen tissue, Cell line, Saliva, Blood | ≥ 500ng | ≥ 20μl | ≥ 20ng/μl | OD260/280 = 1.8 - 2.0 OD 260/230 ≥ 1.7 DIN ≥ 7 |
| gDNA | FFPE | ≥ 800ng | ≥ 20μl | ≥ 20ng/μl | Main band of > 1,500 bp (Q Score > 0.5 DIN ≥ 4 |
Please note that these requirements serve only as a guide. Please contact us for further assessment if your samples do not meet the requested amounts.
- Data Quality Control: Filtering reads with adapter
- or low-quality sequence data
- Alignment to reference genome using BWA
- Summary statistics of sequencing depth and coverage
- Germline variant calling based on best practices
- Somatic variant calling for tumor alone and/or tumor-normal paired based on best practices
- Full annotation services
- Joint calling for SNPs and
- Indels
- Tumor Purity
- Tumor Mutation Burden
- Microsatellite Instability
- Many other options upon request