Multiomics
Spatial Transcriptomics
Stereo-seq (FFPE)
Spatial Transcriptomics powered by Stereo-seq (SpaTial Enhanced REsolution Omics-sequencing) for FFPE tissues retains the advantages of Stereo-seq (subcellular resolution and large field of view), while expanding the application to FFPE tissues.
The novel Stereo-seq FFPE chip utilizes spatially-barcoded random probes to efficiently capture mRNA, long non-coding RNA (lncRNA) and microbial RNA in situ. Coding and non-coding RNA are spatially profiled at nanoscale resolution to reveal unprecedented insights.
Formalin-Fixed Paraffin-Embedded (FFPE) Samples
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Single Tissue Section, Multiple Information Layers
Sample preparation
Staining and imaging
Permeabilization, in situ RNA capture and reverse transcription
Library construction and sequencing
Bioinformatics analysis
| Sample Type | Available chip sizes and recommended data quantity# per chip | Sequencing Platform | Turnaround Time |
|---|---|---|---|
| FFPE Sample* | 1 cm x 1 cm (3 billion raw reads) | DNBSEQ-T7 | 4-6 weeks from successful tissue sectioning and mounting to FASTQ delivery |
# The actual data output depends on sample quality
- FASTQ Files
- Stained Tissue Image(s)
- Barcode to Position Mapping File
- QC Statistics
- QC statistics and Spatial Barcodes Maps
- Sequence Alignment Maps
- Gene Count Matrix
Host and microbial
- Spatial Gene Expression Cluster Analysis
- Spatial Copy Number Variation
- Trajectory Inference
- Cellular Neighbourhood Analysis
- Co-localization Analysis
- Many other options upon request